UM E-Theses Collection (澳門大學電子學位論文庫)
Title
Analysis of hydrophilic components in water extract from Polygonum multiflorum using micellar electrokinetic chromatography
English Abstract
Polygonum multiflorum Thunb, He-Shou-Wu in Chinese, is a widely used traditional Chinese medicine as a tonic and anti-aging agent. Since its major application form is water decoction, it was aimed to develop a simple and fast analytical method to analyze its hydrophilic bioactive components in its water extract. Therefore, pressurized liquid extraction and short-end injection micellar electrokinetic chromatography (MEKC) method were established for simultaneous determination of hydrophilic components including one alkaloid (hypaphorine), one stilbene (2,3,5,4’-tetrahydroxystilbene 2-O-β-D-glucoside), and five polyphenols (proanthocyanidin B1, proanthocyanidin B2, catechin, epicatechin, and gallic acid) in water extract of He-Shou-Wu. Method development was systematically investigated, the influence of parameters, such as pH, capillary temperature, separation voltage, concentration of phosphate, SDS and HP-β-CD, on the analysis were carefully investigated. Optimum separation was obtained within 14 min by using 50 mM phosphate buffer containing 90 mM SDS and 2% (m/v) HP-β-CD (pH 2.5) at 15 kV and 20 oC. All calibration curves showed good linearity (R2>0.9978) within test ranges. The overall LOD and LOQ were lower than 2.0 μg/mL and 5.5 μg/mL, respectively. The RSDs for intra- and inter-day of seven analytes V were less than 3.2% and 4.6%, and the recoveries were 97.0-104.2%. The validated method was successfully applied to the analysis of He-Shou-Wu samples collected from different regions of China, which is helpful for the quality control.
Issue Date
2013
Author
Lao, Ka Meng
Faculty
Institute of Chinese Medical Sciences
Degree
M.Sc.
Subject
Polygonum
Medicinal plants -- China -- Analysis
Materia medica -- China -- Analysis
Chinese Medicinal Science -- Institute of Chinese Medical Sciences
中藥學 -- 中華醫藥研究院
Supervisor
李紹平
Library URL
b3091635
Files In This Item:
TOC & Abstract
Full-text
Location
1/F Zone C
Supervisor
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