The therapeutic effects of herbal medicine are depended on their stable quality of the raw materials, which may be affected by the locations, collection time and storage conditions of the herbs. Therefore, it is very important and necessary to evaluate the quality consistency of herbs used in clinic, which are major form used in clinical practices. In this paper, 56 samples of Angelica sinensis and 58 samples of Ligusticum chuanxiong, collected from 58 provincial hospitals in China, were qualitatively and quantitatively analyzed using HPIC and their quality consistency was evaluated using "Similarity Evaluation System for Chromatographic Characteristics of Traditional Chinese Medicine" (SES), Clustering Analysis and Principal Component Analysis. In SES analysis, the similar coefficients of Angelica sinensis and Ligusticum chuanxiong were distributed in the ranges of 0.989 _ 0.744 and 0.999- 0.909, respectively. However the SDs of ferulic acid, coniferyl ferulate, E/Z-ligustilide, and E/Z-butylidenephthalide in 56 samples of Angelica sinensis and 58 samples of Ligusticum chuanxiong were distributed from 26.7% to 84.0% and 23.2% to 63.8%, respectively, which suggested that there were differences in quality of these herbal medicines. Clustering Analysis based on the major compounds in the materials showed that both Angelica sinensis and Ligusticum chuanxiong samples were divided into two major groups with the sample numbers of each larger group were 40 and 37, and accounting for 71.4% samples of Angelica sinensis and 63.8% samples of Ligusticum chuanxiong, respectively. The RSDs of ferulic acid + coniferyl ferulate, and Z-ligustilide of samples in the larger group of these two species were less than 40%. Principal Component Analysis was also performed and 2D-projection plots of Principal Component Analysis for 56 samples of Angelica sinensis and 58 samples of Ligusticum chuanxiong showed the samples were also divided into different groups according to their distribution. Based on the samples located in the larger groups, RSDs of ferulic acid + coniferyl ferulate, and Z-ligustilide in 89.3% samples of Angelica sinensis and 60.3% samples of Ligusticum chuanxiong sample were less than 40%. Considering the data mentioned above, RSDs of ferulic acid + coniferyl ferulate, and Z-ligustilide in, at least, 70% samples of Angelica sinensis and 60% samples of Ligusticum chuanxiong used in clinic in China were less than 40%, which is helpful to ensure the safety and efficacy of medication. Decoction is the major administration form of Chinese herbs. Little Z-ligustilide could be extracted by water decoction, though ultrasonic extraction with methanol-formic acid (95:5 had good selectivity for weak polarity components in Angelica sinensis and Ligusticum chuanxiong. It is interesting that the RSD of this compound in water decoction was less than that in ultrasonic methanol-formic acid extracts. Conifery! ferulate, at least could be partially transformed into ferulic acid during the decoction, was hardly detected in the water decoction extract. So the total amount of ferulic acid in Angelica sinensis and Ligusticum chuanxiong by decoction was higher than those extracted by ultrasonication, but its RSD of content in water decoction was higher. 

目前，開於中藥材中的化學成分與中醫臨療效之間的研究表明，中藥的臨床療 效極大程度上基於中藥材品質的稳定可靠。而中藥材的品質受到諸多因素的影響， 例如藥材的品種，藥材種植過程中的自然環境、探收季節，以及保存條件等。臨床 使用的中藥直接爲病人所服用，對中醫臨床療效有實際影響，要保證中藥臨床療效 的穩定可靠，針對臨床使用的中藥材的品質一致性訴價顯得猶爲重要。 本論文通過對探集自全國 26 個省市 58家省級醫院/中醫院的臨床使用的 56份 當歸樣品及 58份川芎樣品進行 HPLC 定性定量分析，應用中藥指紋圖譜相似性評價，聚類分析和主成分分析，從中藥材 “性”與“量’相似性兩個方面對藥材的品質均 一性進行研究，以揭示目前臨床使用的中藥材的品質現狀，同時也針對中藥材的品 質評價方法提出若干探索性的意見。 結果顯示，“中藥色譜指紋圖譜相似度評價系統” 所給出的檠材相似度係數得 分較高，56份當歸樣品的相似度系數在 0.989～0.744 之間；58份川芎樣品的相似 度係數在 0.999~0.909 之間。但藥材的定量分析結果顯示，56 份當歸樣品中所含 的阿魏酸，阿魏酸松柏酯，Z槁苯內酯，Z/E -3-丁烯基苯酞的 RSD 值波動範圍是 從26.7%到84.0%，而58份川芎樣品中所含的有效成分的 RSD 值的波動範圍從 23.2 ％到 63.8%，說明藥材中所含的有效成分在含量上存在不均一性。 基於主要成分的聚類分析顯示，當歸與川芎藥材都被分爲兩個組，其中較大的 組分别包含 40 個當歸樣品和 37 個川芎樣品，分別占各自藥材總樣本量的 71.4%和 63.8%，並且組中樣本所含的阿魏酸＋阿魏酸松柏酯和 子搞本內酯的 RSD 值小於 40 % • 主成分分析的二維散點圖顯示了藥材的分佈情況，同時也提示了樣本間的相似 性。89.3%的當歸樣品與 60. 3%的川芎樣品在二維散點圖中的分佈較爲集中，定量分 析結果顯示，分佈較為集中的當歸與川芎樣品中所含的阿魏酸＋阿魏酸松柏酯和乙 槁本內酯的 RSD 值小於 40%° 結合以上分析結果，70%以上當歸藥材和 60%以上川芎藥材樣品所含阿魏酸＋阿 魏酸松柏酯和 二槁本內酯的 RSD 值小於40%。該結果反映了臨床使用中藥材的品質 均一性情況。湯劑是中藥臨床給藥的主要形式。本研究也針對不同提取方法所得到的藥材提 取物的品質均一性也進行了評價，結果顯示，傳統水煎煮對於當歸及川芎中的揮發 性成分的提取效率較低，但两種藥材 10 個樣品水煎液中所含的2一蒿苯內酯的 RSD 值也减小。在兩種藥材的水煎煮液中，均未能檢測到阿魏酸松柏酯，而阿魏酸的含 量卻有所增加，但阿魏酸含量的 RSD 值也有所增加，可能與煎煮過程中阿魏酸松柏 酷轉化爲阿魏酸的不均一性有關，提示不同的提取方法也對中藥的品質均一性產生 影響