澳門大學碩士論文 Abstract The pharmacological studies indicated that the essential oil from Curcuma showed strong anti-tumor and anti-thrombus bioactivities. In this thesis, preparative isolation and purification of compounds from essential oil of Curcuma wenyujin using high performance centrifugal partition chromatography and separation and identification the chemical constituents of the essential oil of C. kwangsiensis were studied. There are mainly three chapters for the thesis. Chapter I introduced the background and strategy for the present study. It is a review for the study on chemical constituents and pharmacologic actions of the essential oil of Curcuma and so on. Chapter II mainly contained two aspects. In one part, it introduced the research on separation and identification the chemical constituents of the essential oil of C. kwangsiensis and C. wenyujin. In another part, it introduced the experiment on preparative isolation and purification of compounds from essential oil of C. wenyujin using high performance centrifugal partition chromatography. Chapter III contained the conclusion and discussion for the results of the study, the results indicated that was practical and feasible for the chemical study of the essential oil from Curcuma. Key words: Curcuma wenyujin; Curcuma kwangsiensis, essential oil; high performance centrifugal partition chromatography (HPCPC); sesquiterpenoid; monoterpenoid IV

澳門大學碩士論文 摘要 藥理研究表明莪術揮發油具有明顯的抗腫瘤、抗血栓等作用。本論文報導 了用高效逆流色譜法分離和純化溫義術(Curcuma wenyujin YH.Chen et C.Ling)揮 發油以及廣西術(Curcuma kwangsiensis S.G.Lee et C.F.Liang) 揮發油的分離和 結構鑒定. 本論文主要包括三個章節。 第一章介紹本課題的研究背景並提出本文的研究方法, 對 術揮發油的化 學成分和藥理作用等的研究進展進行了綜述。 第二章主要分2個部分,分別介紹了對廣西莪術和溫 術兩種揮發油的化學 分離和結構鑒定以及用高效逆流色譜法分離和純化溫莪術揮發油。 第三章對實驗結果進行了總結和討論,並說明了研究的實用性和可行性,體 現了研究的實際意義。 關鍵字:溫莪術,廣西莪術,揮發油,高效逆流色譜,倍半,單 ΠΙ