澳门大学硕士学位论文 Abstract Radix Angelicae sinensis (Danggui in Chinese) is one of the most important traditional Chinese medicine, which has been used for tonifying blood, treating irregular menstruation and amenorrhoea for over 2000 years in China. Many analytical methods such as HPLC-UV, GC-MS and CE have been developed for quality control of Danggui. Ferulic acid is commonly used as the marker. However, ferulic acid is rarely found in the free form in plants. In previous study, coniferyl ferulate was found in Angelica sinensis. There are a few reports on its physical and chemical properties, pharmacological activities and the content in Danggui. This study focused on the preparation and pharmaceutical activity of coniferyl ferulate in Danggui. 1. The solvent extraction method for enrichment of coniferyl ferulate form Danggui oil, and a HPCPC method for purification of coniferyl ferulate were developed. 2. A rapid HPLC-DAD method was developed for the determination of coniferyl ferulate in Danggui and Chuanxiong. 3. The antiplatelet aggregation activity of coniferyl ferulate was tested in vitro. 4. Coniferyl ferulate in 13 species medicinal plants from Umbelliferae family were screened. II

摘要 ABSTRACT 缩略词对照表 第一章 当归中阿魏酸及阿魏酸松柏酯研究进展 I IV 1 第一节 阿魏酸研究进展 1 第二节 阿魏酸松柏酯研究进展 6 第二章 当归中阿魏酸松柏酯分离与纯化, 10 第一节 硅胶柱层析法自当归油中分离纯化阿魏酸松柏酯 10 第二节 溶剂提取/萃取法制备阿魏酸松柏酯富集物 21 第三节 高效离心分配色谱(HPCPC)纯化阿魏酸松柏酯 25 第三章 HPLC-DAD测定当归与川芎中阿魏酸松柏酯含量 31 第一节仪器与试药 31 第二节 方法与结果 32 第三节讨论 38 第四章 阿魏酸松柏酯的抗血小板聚集活性研究 43 第一节仪器与材料 43 第二节方法 44 第三节 结果 46 第四节讨论 52 第五章.13种常用伞形科中药含阿魏酸松柏酯筛选 56 第一节仪器与试药 56 第二节方法 58 第三节 结果与讨论 58 结语 64 附图1 阿魏酸松柏酯1H-NMR和13C-NMR谱图 65 附图2 阿魏酸松柏酯在不同溶剂中稳定性的HPLC色谱图, 71 澳门大学硕士学位论文 附图3 不同溶剂提取/萃取得到的阿魏酸松柏酯富集物HPLC图 73 附图4 不同来源当归与川芎HPLC色谱图 75 附图5 伞形科中药的HPLC,总离子(TLC)色谱图,选择离子色谱图及相应的 质谱图 79 参考文献 86 致谢 93 论文期间发表及待发表文章, 94