STUDY ON THE TECHNOLOGY OF EXTRACTION OF THE BIO-ACTIVE CONSTITUENTS AND THЕ МЕТНOD OF QUALITY CONTROL OF RHIZOMA CHUANXIONG ABSTRACT Szechwan Lovage Rhizoma is the dried rhizome of Ligusticum chuanxiong Hort. Chuanxiong is one of the traditional Chinese medicine. Chinese medicine which has the actions of promoting blood flow and qi, dispersing wind and relieving pain. Clinically, it is always used for the treatment of menstrual disorders, amenorrhea, dysmenorrheal, various types of pain such as abdominal pain, pricking pain in the chest and costal regions and pain due to traumatic injury, swelling, headache and arthritis. Modern research usually concentrate on the pharmacological activity of the active crude extracts of Chuanxiong and their clinically use. However, chemical compositions and their therapeutic effects are rarely reported. Therefore, it is essential to find out the active ingredients which match with its therapeutic uses, and use them as the markers for the quality control of Chuanxiong. The research was divided into two aspects - extraction of active ingredients and study of the quality control methods for Chuanxiong. The new methods could be set as reference standards for quality control of -2- Chuanxiong. In the extraction of active components, ASE was used to extract three active ingredients, ligustrazine, ferulic acid and Z-Ligustilide in Chuanxiong. Different conditions were studied in order to optimize the extraction methods. Finally, the results obtained from ASE were compared with the traditional extraction methods for Chuanxiong. In the active ingredient analysis, HPLC and GS-MS were used. Optimized conditions were studied for both methods and protocols were set. Then, 14 species of Chunaxiong with various origins were analyzed with the GC-MS protocol. Finally, fingerprints of Chuanxiong were established. All the studies show that GC-MS method is accurate, reproducible, easy to use and quick for the quality control of Chuanxiong with various origins. Key words: Rhizoma chuanxiong, ligustrazine, ferulic acid, z-ligustilide, ASE, HPLC, GC-MS, quality evaluation

川芎活性成分提取工藝及品質評價方法研究 中文摘要 中藥川芎為傘形科藁本屬植物川芎 Ligusticum chuanxiong Hort. 乾燥的根莖, 是常用的傳統中藥,傳統中醫認為:川芎具有活血行氣,祛風止痛的功效,常用 於女性月經不調,閉經痛經,產後淤滯腹痛,以及胸肋刺痛,跌撲腫痛,頭痛, 風濕痹痛等等。 現代的研究比較多的集中在川芎總提取物的藥理活性以及這些活性部位群在 臨床治療上的作用,而對川芎的藥效物質基礎——化學成分和藥效,作用機理以 及這兩者之間的關係則較少報導,因此,尋求與中醫用藥大體相吻合的藥效物質 基礎來控制中藥川芎的品質是我們研究的關鍵。 本課題主要從川芎中活性成分的提取工藝和品質評價方法研究兩個方面入 手,對川芎中有效成分的品質控制的方法學研究做深入的探討,以期為川芎藥材 的品質評價的規範化提供借鑒和參考。 在川芎有效成分的提取優化的研究中,我們將首次採用加速溶劑提取技術 (ASE)對川芎 、阿魏酸和藁本內酯三種有效成分的提取方法進行了優化選擇, 並與常規的提取方法進行比較,最終建立了科學的、合理的、規範的提取方法。 在對川芎有效成分的分析研究中,我們分為兩步:首先通過對色譜條件的優 化,建立了川芎有效成分的HPLC,GC-MS 檢測的最佳條件;然後我們通過對來 自14個不同來源的川芎藥材樣品的分析,初步建立了川芎藥材的GC-MS 的指紋 圖譜,通過對實驗結果分析比較,結果表明,本方法精密度、重現性良好,可簡 便快速的鑒別不同來源的川芎藥材。 關鍵詞: 川芎 川芎 阿魏酸 高效液相色譜 氣相色譜-質譜聯用 -1- 藁本內酯 加速溶劑提取 品質評價