Rhizoma Cyperi, belonging to family Cyperaceae, is the rhizome of the plant Cyperus Rotundus L. It is maily distributed in Shangdong, Zhejiang, Hunan and Henan province in China. And the products from Shangdong province and zhejiang province is better. It can release stagnation of the liver Qi characterized by distending pain in the chest, hypochondria and epigastrium, indigestion, feeling of stuffiness in the chest and epigastrium, abdominal colic, distending pain in the breast, menstrual disorders, amenorrhea of dysmenorrhea. It was traditional used for soothing the liver and regulating flow of Qi, regulating menstruation to relieve pain. Cyperus Rotundus contains 0.65%~1.4% volatile oil. Hydro distillation (HD) is the traditional method to extract the oil. Supercritical Fluid Extraction (SFE), a new metho, was widely used in many fields. A few studies about the extraction of Cyperus Rotundus had been reported. Pressurized Solvent Extraction (PSE) is a method extracted by solvent in high temperature and pressure. It has not been used in Cyperus Rotundus study before. This thesis consists of four chapters. Chapter 1 is the literature review on Cyperus Rotundus, which includes the recent development on chemical compositions, pharmacological studies and extraction methods of Cyperus Rotundus. Chapter 2 is about the separation and confirmation of chemical compound in volatile oil. One compound was isolated and identified by MS and NMR data. Purity test showed that the compound can be used as chemical standard. The qualitative and quantitative analysis of Cyperus Rotundus by using GC-MS was showed in chapter 3. Chapter 4 focused on the study of extraction methods. PSE and SFE were optimized. Finally, comparisons were made between HD, PSE and $FE. The result indicated that the extraction efficiency of PSE is better than HD and SFE. Key words: Cyperus Rotundus a-cyperone Hydro Distillation Pressurized Solvent Extraction Supercritical Fluid Extraction Gas Chromatography-Mass Spectrum

香附，異名雀頭香、莎草根等，為莎草科植物莎草(Gyperus Rotundus L.)的乾燥根莖，主產於山東、浙江、湖南、河南等地，以山東產者稱“東香附”，浙江產者稱“南香附”品質較佳。本品味辛、微苦、微甘、平，歸肝、脾、三焦經。香附為常用中藥，具有行氣解鬱，調經止痛的功效，用於肝滞，胸、脇、脘腹脹痛，消化不良，胸脘痞悶，寒疝腹痛，乳房脹痛，月經不调，經閉痛經等。 香附中含有揮發油0.65~1.4%，國内外對香附研究主要集中在揮發油的化學成分方面，提取工藝的系统研究较少。香附揮發油的提取方法有傳统的水蒸氣蒸餾法，CO2超臨界萃取技術是近年來應用比较廣泛的技術，也有提取香附揮發油的研究報導；加壓溶劑提取是一種在高溫和壓力條件下用有機溶劑萃取的方法，其突出的是有機溶劑用量少、快速、提取率高，目前尚未有應用於香附揮發油提取的相關報導。本研究是對香附揮發油不同提取工藝(水蒸氣蒸餾，CO2超臨界萃取，加壓溶劑提取)進行優化，並将三种提取方法進行比较，從中選出香附揮發油的最佳提取方法。本文章分為四部分:第一章為文獻綜述，内容包括香附的化學成分、藥理研究和揮發油提取方法三個方面。 第二章為a-香附酮的分離製備，探用矽膠柱層析，中壓柱色譜，製備型高效液相色譜從市售香附油中分離得一個化合物，經MS，1H-NMR，13C-NMR等確證，鑒定為a-香附酮，HPLC檢查纯度達到95%以上，適合作为品質控制的對照品。第三章是香附揮發油的GC-MS分析，GC-MS通過程序升溫使各主要组分在毛細管柱中得到分離，對相關组分作定性，並以特徵離子進行半定量。第四章是香附揮發油提取方法的比較，分別運用水蒸氣蒸餾、CO2超臨界萃取、加壓溶劑提取從香附中提取主要成分，並優化提取條件，對三种提取方法作比较。綜上所述，本研究從香附油中分離得到一個主要成分(a-香附酮)，並進行了標準化研究，建立了以PSE提取、GC-MS分析测定香附中主要成分的定量方法，並三種提取方法進行優化及比較，結果顯示PSE提取香附藥效成分簡便快速，提取效率最高。 關鍵詞:香附 α-香附酮 水蒸氣蒸餾 CO2超臨界萃取 加壓溶劑提取 氣相色譜-質譜聯用