Radix Astragali, a kind of Traditional Chinese Medicine (TCM), is categorized under "bu zhong yi qi" from the perspectives of TCM. Quality control is the first key step in order to identify the mechanistic action of the herb. Therefore, the separation of reference chemical standards and, the quantitative and qualitative analysis of the chemical standards in the herb are very important for investigation. In China pharmacopoeia 2005, only Astragaloside IV was considered to be the standard marker for quality control on Radix Astragali. As we know, the effect of Traditional Chinese Medicine (TCM) is the result of combinatory actions of many compounds working together. So, single chemical marker can not totally reflect the quality of TCM. Manufacturing or isolation of sufficient varieties and quantities of reference chemical standards is one of the key step for multiple-marker quality control method. So, we decided to isolate the main chemical components in Radix Astragali at the initial of this study, and finally had isolated five compounds which structure were identified by UV, MS and NMR. Then, the isolated chemical compounds together with some commercial available chemical markers have been used to serve as reference standards for analysis of the chemical composition of Radix Astragali by HPLC-ELSD method. This study consisted of the following parts: Section 1: Review of the Radix Astragali. The current situation and outlook of the research on Radix Astragali were summarized by review of literatures. Section 2: Separation and isolation of the main components from Radix Astragali. Using the silica gel method, MPLC method, pre-HPLC method and recrystallization, five different compounds namely (GaR, 11aR)-9, 10-dimethox y-3-hydroxypterocarpan, calycosin, fomononetin, -sitosterol and Bu2 (not identified yet) with high purity have been isolated and identified for quality control of the herb. Section 3: Quality control of several compounds in Radix Astragali with quantitative and qualitative chemical analysis methods. Using the HPLC -ELSD column switching, several reference chemical standards including Astragaloside, isoflavone and some other components in Radix Astragali were analyzed. Section 4: Pharmacological study of Radix Astragali. The effects of the fraction, extracted by ethyl acetate and normal butanol, on HUVEC cells proliferation have been investigated.

黄芪作爲一味傅統的補虛益氣類中藥，任今天正在被現代的分析手段所研 筅。其品質控制方法的完善是關明各類物質結構、作用機理的基礎。因此，標誌性成分標準品的分離、多種成分的定性定量分析是研究該種樂材的基本過程。中國藥典2005版對於黃芪的品質控制方面，只規定了黄芪甲街的含量不低於 0.03%。由於中藥的效應為多種成分綜合作用的結果，因此，單一化學指標難以 客觀反映黄芪藥材的品質；而多指標品質控制的關鍵之一是必須具有足夠的中藥 化學對照品。爲此，我們首先對黄芪中的主要化合物進行了分離純化，得到5個 化合物，並以UV，MS和NMR 雄證了其結構。在此基礎上，建立了中藥黄茋 HPLC-ELSD的定性定量分析方法。 本課題是澳門大學中華醫藥研究所中藥學專業碩上研究生課題。在一年的實 驗過程中，我們的工作主要包括了以下內容： 第一部分：對現階段黃芪的研究進行總結。主要包括化學成分和藥理活性兩 個方面，通過近一段時期國内外有關黃芪的文獻報導，總結其質控方法和藥理活 性特點，為將來的研究打下基礎。第二部分：黄芪藥材中幾種主要成份的提取分離。通過矽膠柱層析分離、中 壓柱層析分離、製備高效液相色譜、反復重結晶等方法分離到了5個並鑒定 了4 個具有較高純度的化合物：3-羥基-9，10-二甲氧基紫檀烷，毛蕊異黃酮，芒柄花素，B-穀甾醇，爲成分的分析或藥理作用的研究提供物質基礎。 第三部分 ：中藥黄芪幾種主要成份的品質控制，包括定性鹽别和定量測定。 主要通過上一階段實驗分離得到的對照品和其他一此;標準品，探用HPLC -ELSD 聯用以及柱切換的方法來同時測定黄芪中的皂苷、異黃酮等多類成分，為今後的 黃芪質控技術的更新發展提供參考。第四部分：黃芪兩種粗提物的細胞樂理學初步研究，王要使用前階段分離的 粗提物對血管內皮細胞(HUVEC)給藥，然後測定其一定指標的變化，進而爲 黄芪對人體的作用機理的閘明提供一定的依據。