Cablin Patchouli Herb is the dried aerial part of Pogostemon cablin (Blanco) Benth. (Fam. Labiatae). The tastes: pungent, warm. Meridian tropism: spleen, stomach, and lung. The plant has been used as Chinese herbal medicine to remove dampness, stop vomiting and stimulate the appetite, and relieve summer-heat. It is used for the treatment of dampness obstruction, stomach distension and vomit, fatigue caused by summerheat-damp, uncomfortable chest depression, cold-damp closing summerheat, vomit and diarrhea accompanying abdominal pain, headache caused by nasal obstruction. It is a common herb that widely used in digestive system diseases. Herba Pogostemonis contains about 2-2.8% volatile oil which is the main effective part of the plant. In this study, two active components (patchouli alcohol and pogostone) were separated and isolated from patchouli oil. The purity of each compound is up to 98%. The quality control system (GC/MS) of Herba Pogostemonis was studied based on patchouli alcohol and pogostone. And 18 samples of Herba Pogostemonis, obtained from different places, and Agastache rugosa, were compared by using GC-MS. This thesis is divided into five chapters: Chapter 1 is the literature review of the studies on volatile components of Pogostemon cablin containing the following parts: chemical constituents, extraction and separation skills, quality control methods and pharmacology. Chapter 2 is about the preparation and identification of reference compounds. Two crystals were obtained from patchouli oil with silica gel column chromatography, extracting and preparative HPLC. The purity of each compound was checked by TLC and HPLC and was found to be over 98%. By comparing the UV, MS, 'H-NMR and 13C-NMR spectra, the two crystals were identified as patchouli alcohol and pogostone, respectively. It is reported that patchouli alcohol has a regulatory action on enteron and spasmolysis of smooth muscle, and pogostone has an antifungal action. These compounds were used as standards for both qualitative and quantitative analyses, which are very important to the establishment of multi-marker quality control system for Herba Pogostemonis. Chapter 3 focuses on the establishment of GC/MS analytical methods for the quantification of these two components in Herba Pogostemonis. In GC/MS method, target ion quantification was used after the separation by temperature gradient program. 18 Chinese Guang Huoxiang samples (Herba Pogostemonis) were obtained and used in the studies. The comparisons among different kinds of Herba Pogostemonis from different habitats and Agastache rugosa were made by using the GC/MS methods. The GC/MS methods were found to be simple, accurate and reproducible. On the other hand, pressurized liquid exraction (PLE) conditions were optimized. Finally, comparisons were made among PLE, ultrasonic extraction (UE), Soxhelt's extract (SX) and stream distillation (SD) which is a traditional extraction method for patchouli oil. In chapter 4, hierarchical clustering analysis based on characteristics of 10 investigated peaks in GC profiles showed that 18 samples could be divided into three main clusters, patchouliol-type, pogostone-type and the third one between the two chemotypes. The simulative mean chromatogram for the three types Herba Pogostemonis was generated using the Computer Aided Similarity Evaluation System. The fingerprint can help distinguish the substitute or adulterant, and further assess the differences of Herba Pogostemonis grown in various areas of China. And Chapter 5 is an overall conclusion. substitues-risorIs / as- et In conclusion, two pure compounds were isolated from patchouli oil and were identified. Both compounds were previously reported to be pharmacologically active. New GC/MS analytical methods for quantification of the two compounds were also established in this study. The accurate quantification may provide a very useful method for the quality control of Herba Pogostemonis and its related products. The fingerprint can help distinguish the substitute or adulterant, and further assess the differences of Herba Pogostemonis grown in various areas of China Keywords: Pogostemon cablin; volatile oil; reference compounds; quality control; GC-MS fingerprint

廣藿香爲唇形科刺蕊草屬植物廣藿香 Posostemon cablin (Blanco) Benth.的乾燥地上部分。其味辛，微溫，歸脾、胃、肺三經，具有芳香化 濁、開胃止嘔、發表解暑的功能，用於濕濁中阻，脘痞嘔吐，暑濕倦怠， 胸悶不舒，寒濕閉暑，腹痛吐瀉，鼻淵頭痛，是消化系統疾患及暑濕時合 之常用中藥。 廣藿香中約含 2-2.8%揮發油，是其主要有效部位。本研究從廣藿香油 中分離出廣藿香醇、廣藿香酮内種活性成分，純度均達到定量用對照品要求。在此基礎上，建立了可對廣藿香中該两種成分同時定量的 GC/S 分 析方法。並對 18個不同產地的樣品以及另一種藿香屬植物藿香進行了比 輘。本文分爲五章：第一章爲交獻綜述，內容包括廣藿香的化學成分、提 取分離、質量控制、藥理四個方面。 第二章爲廣藿香油對照品的分離製備及標化。探用硅膠桂層析，萃取分離結合製備型高效液相色譜從市售廣藿香油中分離得到两個結晶性 化合物，經UV 光譜、MS 、IH-NMR、13C-NMR 等確證，分別喬廣藿香醇 和廣藿香酮，HPLC 檢查純度均達到 98%以上，交獻報導廣藿香醇具有調 節消化道功能和胃腸丕滑肌解痙作用，廣藿香酮具有抗菌的作用。這一部 分工作解決了廣藿香缺乏化學對照品、控制指標單一的問題，爲藥材質量 控制研究奱定了基礎。 第三章進行了廣藿香多指標成分定性、定量研究。廣藿香藥材及製劑 廣藿香爲唇形科刺蕊草屬植物廣藿香 Posostemon cablin (Blanco) Benth.的乾燥地上部分。其味辛，微溫，歸脾、胃、肺三經，具有芳香化 濁、開胃止嘔、發表解暑的功能，用於濕濁中阻，脘痞嘔吐，暑濕倦怠， 胸悶不舒，寒濕閉暑，腹痛吐瀉，鼻淵頭痛，是消化系統疾患及暑濕時合 之常用中藥。 廣藿香中約含 2-2.8%揮發油，是其主要有效部位。本研究從廣藿香油 中分離出廣藿香醇、廣藿香酮内種活性成分，純度均達到定量用對照品要求。在此基礎上，建立了可對廣藿香中該两種成分同時定量的 GC/S 分 析方法。並對 18個不同產地的樣品以及另一種藿香屬植物藿香進行了比 輘。 本文分爲五章：第一章爲交獻綜述，內容包括廣藿香的化學成分、提 取分離、質量控制、藥理四個方面。 第二章爲廣藿香油對照品的分離製備及標化。探用硅膠桂層析，萃取分離結合製備型高效液相色譜從市售廣藿香油中分離得到两個結晶性 化合物，經UV 光譜、MS 、IH-NMR、13C-NMR 等確證，分別喬廣藿香醇 和廣藿香酮，HPLC 檢查純度均達到 98%以上，交獻報導廣藿香醇具有調 節消化道功能和胃腸丕滑肌解痙作用，廣藿香酮具有抗菌的作用。這一部 分工作解決了廣藿香缺乏化學對照品、控制指標單一的問題，爲藥材質量 控制研究奱定了基礎 第三章進行了廣藿香多指標成分定性、定量研究。廣藿香藥材及製劑通常以廣 香醇為對照品進行鑒別及含量測定,本文以自行分離得到的两種活性成分為指標,建立了可對兩種成分同時定量的GC/MS 分析方法。GC/MS 通過程序升溫使各組分在毛細管柱中得到分離,並以特徵離子定量。這種方法簡便快捷、準確可靠、重現性好。並對多個產地的廣 香以及 香(另一个 香属植物)以GC/MS 方法進行含量測定,並進行了比較,發現不同產地的藥材中廣 香油成分的含量有比較明顯的差別。本文首次採用加壓溶劑提取技術(PLE)提取廣 香中的藥效成分,對提取條件進行了優化,並用於樣品溶液的製備;對 ASE 與超聲提取,索式提取以及傳統的水蒸氣蒸餾法進行了比較,ASE 簡便快速、提取效率高。第四章基於18個樣品的10个特徵峰的聚類分析,將18個樣品分為三個类型-廣 香醇型、廣 香酮性以及基於兩者之間的過渡型。並通過電腦類比相似性評價系統對三個類型進行了相似性的評價,建立了指紋圖譜。指紋圖譜的建立有助於區分替代品和偽品,也更有助於對中國不同地區的廣 香進行評價。第五章對整個研究工作進行總結與討論。綜上所述,本研究從廣 香油中分離得到兩個活性成分(廣香醇、廣 香酮),並進行了標準化研究。以自製對照品為指標成分,首次建立了 PLE 提取-GC/MS 測定廣 香中两種活性成分的含量,為廣 香藥材及製劑的質量控制提供了方法學依據。指紋圖譜的研究有助於區分替代品和偽品,也更有助於對中國不同地區的廣 香進行評價。 關鍵字:廣 香;揮發油;對照品;質量控制;GC-MS 指紋圖譜