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Macau Periodical Index (澳門期刊論文索引)

Author
Zhang, Xiuzhen; Li, Ying
Title
Relationships of estrogen receptor gene polymorphism and bone metablism in postmenopausal women
Journal Name
澳門醫學雜誌
Pub. Info
2003年3月26日, Vol.3, No.1, pp. 6-9
Keyword
Estrogen receptor；Gene polymorphism；Osteoporosis；Postmenopausal
Abstract
Objective: To study the relationships among PvuII, XbaI restriction fragment length polymorphism of estrogen receptor gene, bone minoral density and bone turnover in postmenopausal women. Methods: 57 Chinese postmenopausal women who had no diseases affecting bone metabolism were chosen randomly, whose blood and urine samples were gathered. PvuII and XbaI RFLP of estrogen receptor gene were detected by PCR-RFLP. BMDwas measured at Iumbar-spine and proximal femoral by DEXA. At the same time, biochemical markers, suchas osteocalcin, NTX were measured. Results: The frequency of ER genotype is as follow: pp, Pp, PP and xx, Xx, XX. The weight of XX genotype group is heavier than the other two groups, but the difference of bone mass index was not significant. The BMD at femoral shaft of xx genotype was significantly lower than that in Xx and XX genotype. XbaI-RFLP is correlated with BMD at femoral shaft. Blood osteocalcin was higher in XX genotype than xx and Xx genotypes. There was no difference found in PvuII genotypes. Conclusions: XbaI-RFLP of ER gene is a major factor to affect bone metabolism in postmenopausal women, X genotype may help preserving bone mass of femoral, which may acquire by affecting bone turnover. Paragraph Headings： 1. Materials and methods 1.1. Subjects 1.2. BMD measurement 1.3. Osteocalcin(OT)measurement 1.4. NTX measurement 1.5. ER gene polymorphism 1.6. Statistical analysis 2. Results 2.1. ER gene PvuII-RFLP analysis 2.2. Characteristics of the population 2.3. The relationships between genotype and bone metabolic biochemical markers 2.4. Relationships between genotype and BMD 3. Discussion Tables： 1. Genotypes and characteristics 2. The relationships between genotype and bone metabolic biochemical markers 3. Relationships between genotype and BMD Figure： 1. ER gene and PvuII, XbaI RFLP