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Macau Periodical Index (澳門期刊論文索引)
- Author
- Zhang, Pei-lin; Sheng, Hui-qiu; Wang, Xiu-qin; Ma, Sheng-di; Xu, Rui-long; Wang, Su-hua; Jin, Zhen-liang; Zou, Hejian
- Title
- Changes of banked blood erythrocyte acetylcho;omesterase activity and its significance in Rescuing organophosphate intoxication patients
- Journal Name
- 澳門醫學雜誌
- Pub. Info
- 2004年6月26日, Vol.4, No.2, pp. 78-80
- Keyword
- Banked blood; Erythrocyte; Acetylcholinesterase; Insecticide; Organophosphate;Intoxication
- Abstract
- Objectives: To investigate the relationship between the preservation days of banked blood and the activity of erythrocyte acetylcholinesterase(AchE) and to investigate the experimental basis of blood exchange therapy and blood transfusion used for the treatment of severe organophosphate intoxication and intermediate syndrome. Methods: Ten healthy volunteers were included in this study from whom two hundred milliliters of whole blood was collected and stored exactly by the standard od banked blood. One milliliter of whole blood was collected from each blood sample for quick assay of erythrocyte AchE(E-ACHE). This procedure was repeated for 21 days. The assay was accomplished using portable cholinesterase analyzer (SB-YY) and reagent plate provided with the analyzer. Results: Although E-ACHE level fluctuated as preservation days extended, no significant decrease was noted. No siginificant difference between groups was observed when analyzed using the analysis of variance (P>0.05). The relative ratio with fixed base fluctuated between 92 and 98. The linear regression equation of the preservation days of banked blood and E-CHE was not statistically significant(p>0.05). Conclusions: Certain amount of E-ACHE existed in banked blood, Its activity did not significantly decrease in 21 days. This study is of refrential value for blood transfusion and blood exchange therapy. Paragraph Headings： 1. Materials and Methods 1.1. Study objects: Data of banked blood sample 1.2. Methods 1.3. Statistical Analysis 2. Results 3. Discussion Tables： 1. Dynamic change of E-ACHE activity at different preservation time Figures： 1. The relationship between preservation time and E-ACHE activity